Enzymes and Inhibitors
Invitrogen™ UltraPure™ Glycogen
Used as carrier for nucleic acid precipitation in ethanol
Thermo Scientific™ Eco47III (AfeI) (10 U/μL)
The Eco47III (AfeI) restriction enzyme recognizes AGC^GCT sites and cuts best at 37°C in O buffer (Isoschizomers: AfeI, Aor51HI).
Thermo Scientific™ Eco52I (EagI) (10 U/μL)
The Eco52I (EagI) restriction enzyme recognizes C^GGCCG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: BseX3I, BstZI, EagI, EclXI).
Thermo Scientific™ Agarase (0.5 U/μL)
Specifically digests the agarose polysaccharide core, which allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose.
Thermo Scientific™ T4 DNA Ligase Buffer (10X)
Safely ligate samples with this buffer, for use with T4 DNA Ligase and tested for the absence of endo- and exodeoxyribonucleases and ribonucleases.
Thermo Scientific™ I-SceI (10 U/μL)
Cut at TAGGGATAA^CAGGGTAAT sites with I-SceI homing enzyme, which performs best at 37°C in Tango buffer.
Thermo Scientific™ FastDigest AjuI
Cut at ^(7/12)GAA(N)7TTGG(11/6)^ sites with Thermo Scientific™ FastDigest™ AjuI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ UTP Solution (100 mM)
Thermo Scientific™ UTP (uridine 5'-triphosphate) is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Invitrogen™ Red/Green BODIPY™ PC-A2 (1-O-(6-BODIPY™ 558/568-Aminohexyl)-2-BODIPY™ FL C5-Sn-Glycero-3-Phosphocholine) (Ratiometric Phospholipase A2 Substrate)
Measure results in microplate assays or using flow cytometry
Invitrogen™ Anza™ 8 XhoI
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Thermo Scientific™ SgsI (AscI) (10 U/μL)
The SgsI (AscI) restriction enzyme recognizes GG^CGCGCC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AscI, PalAI).
Thermo Scientific™ SsiI (AciI) (10 U/μL)
Cut at CCGC(-3/-1)^ sites with SsiI (AciI) restriction enzyme, which functions best at 37°C in O buffer (Isoschizomers: AciI, BspACI).
Thermo Scientific™ Bsp119I (BstBI) (10 U/μL)
Cut at TT^CGAA sites with Bsp119I (BstBI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AsuII, Bpu14I, BspT104I, BstBI, Csp45I, NspV, SfuIm).
Thermo Scientific™ AloI (2 U/μL)
Cut at ^(7/12-13)GAAC(N)6TCC(12-13/7)^ sites with AloI restriction enzyme, which performs best at 30°C in R buffer.