Enzymes and Inhibitors
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Thermo Scientific™ Bsp1407I (BsrGI) (10 U/μL)
Cut at T^GTACA sites with Bsp1407I (BsrGI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BsrGI, BstAUI).
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ FastDigest BspTI
Cut at C^TTAAG sites with Thermo Scientific™ FastDigest™ AflII (BspTI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Invitrogen™ Anza™ 10 DpnI
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Approved for shipment on Wet or Dry Ice |
|---|---|
| Sensitive to Heat Inactivation | Yes |
| Product Type | Restriction Enzyme Cloning System |
| Enzyme | Dpn I |
| Methylation Sensitivity | Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Anza™ 10X Red Buffer, Anza™ 10X Buffer |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Product Line | Anza |
Invitrogen™ Anza™ 93 HpaII
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Anza™ 11 EcoRI
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Approved for shipment on Wet or Dry Ice |
|---|---|
| Sensitive to Heat Inactivation | Yes |
| Product Type | Restriction Enzyme Cloning System |
| Enzyme | EcoR I |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Anza™ 10X Red Buffer, Anza™ 10X Buffer |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Product Line | Anza |
Thermo Scientific™ Eco147I (StuI) (10 U/μL)
The Eco147I (StuI) restriction enzyme recognizes AGG^CCT sites and cuts best at 37°C in B buffer (Isoschizomers: AatI, PceI, SseBI, StuI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Eco147I (StuI) |
| Methylation Sensitivity | Dcm Methylation-Sensitive,Not Dam Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer B |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest NcoI
The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Nco I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest |
Thermo Scientific™ AluI (10 U/μL)
Cut at AG^CT sites with AluI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AluBI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Alu I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ Bsh1236I (BstUI) (10 U/μL)
Cut at CG^CG sites with Bsh1236I (BstUI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AccII, BspFNI, BstFNI, BstUI, MvnI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Bsh1236I (BstUI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer R |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest PvuI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ Cfr9I (XmaI) (10 U/μL)
The Cfr9I (XmaI) restriction enzyme recognizes C^CCGGG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: TspMI, XmaCI, XmaI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Cfr9I (XmaI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Unique Buffer (10X Buffer Cfr9I (XmaI)) |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FastDigest HinfI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ ApaI (10 U/μL)
Cut at GGGCC^C sites with ApaI restriction enzyme, which functions best at 37°C in B buffer (Isoschizomers: PspOMI).
| Concentration | 10 U/μL |
|---|
Invitrogen™ TrueCut™ HiFi Cas9 Protein (1 μg/μL)
A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut™ HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Form | Liquid |
|---|---|
| Product Type | HiFi Cas9 |
| Purity | ≥90% |
| Concentration | 1 μg/μL |
| Source | E. coli |
Invitrogen™ Anza™ 1 NotI
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Approved for shipment on Wet or Dry Ice |
|---|---|
| Sensitive to Heat Inactivation | Yes |
| Product Type | Restriction Enzyme Cloning System |
| Enzyme | Not I |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | Anza™ 10X Red Buffer, Anza™ 10X Buffer |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Product Line | Anza |